SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameARO10
Systematic nameYDR380W
DescriptionTransaminated amino acid decarboxylase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K588        
2         
3 MULTI    RPN10 Proteasomal Degradation
4K372        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06408
SGD YDR380W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0050177phenylpyruvate decarboxylase activityIDA:SGD.
Biological processGO:0006559L-phenylalanine catabolic processIDA:SGD.
Biological processGO:0006552leucine catabolic processIMP:SGD.
EC number 4.1.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length635
Molecular weight71384

	---------+----------+----------+----------+----------+
MAPVTIEKFV NQEERHLVSN RSATIPFGEY IFKRLLSIDT KSVFGVPGDF   50
NLSLLEYLYS PSVESAGLRW VGTCNELNAA YAADGYSRYS NKIGCLITTY   100
GVGELSALNG IAGSFAENVK VLHIVGVAKS IDSRSSNFSD RNLHHLVPQL   150
HDSNFKGPNH KVYHDMVKDR VACSVAYLED IETACDQVDN VIRDIYKYSK   200
PGYIFVPADF ADMSVTCDNL VNVPRISQQD CIVYPSENQL SDIINKITSW   250
IYSSKTPAIL GDVLTDRYGV SNFLNKLICK TGIWNFSTVM GKSVIDESNP   300
TYMGQYNGKE GLKQVYEHFE LCDLVLHFGV DINEINNGHY TFTYKPNAKI   350
IQFHPNYIRL VDTRQGNEQM FKGINFAPIL KELYKRIDVS KLSLQYDSNV   400
TQYTNETMRL EDPTNGQSSI ITQVHLQKTM PKFLNPGDVV VCETGSFQFS   450
VRDFAFPSQL KYISQGFFLS IGMALPAALG VGIAMQDHSN AHINGGNVKE   500
DYKPRLILFE GDGAAQMTIQ ELSTILKCNI PLEVIIWNNN GYTIERAIMG   550
PTRSYNDVMS WKWTKLFEAF GDFDGKYTNS TLIQCPSKLA LKLEELKNSN   600
KRSGIELLEV KLGELDFPEQ LKCMVEAAAL KRNKK                   635

	* Ubiquitinated lysine is denoted as red color.