SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameARC35
Systematic nameYNR035C
DescriptionActin-related protein 2/3 complex subunit 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP53731
SGD YNR035C
Gene Ontology (GO)
Cellular componentGO:0005885Arp2/3 protein complexIDA:SGD.
Cellular componentGO:0005829cytosolIPI:SGD.
Molecular functionGO:0005198structural molecule activityIMP:SGD.
Biological processGO:0030036actin cytoskeleton organization and biogenesisIGI:SGD.
Biological processGO:0000226microtubule cytoskeleton organization and b...IGI:SGD.
Biological processGO:0000001mitochondrion inheritanceTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
324Phosphoserine.

Sequence Information
Sequence length342
Molecular weight39566

	---------+----------+----------+----------+----------+
MLHLQPQNLL IQKTLNEAIE ALRKGSPLTM DRIVSDFDYT TYHISNTAED   50
KSILLLSVKT KAWVSVSECQ LDGSLTLLKF LADHYSSLGG VTIPSEVEPG   100
YDYTLQITLA ELVQESILQL SVLKTIILSF PFELAISKFI ELSQQQPAPV   150
EAEITGGEVA ANGDNTLFTI KYRDEENIFI KPSNDRVTII FETIFQDETD   200
KIFGKVFLQE FVDARKRNRQ IQSAPQVLYS HEPPLELKRL YQPPKVAEQS   250
RRFITFVLFP RHFQTKELQF HSICQLTLFR NYFHYHIKCS KAYMHSRMRF   300
RVDSFIKVLN RAKVDEDDEN DELSAEGRQQ ARRTFTGRKI VY           342