SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAPA1
Systematic nameYCL050C
AliasDTP1
DescriptionADP-sulfurylase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP16550
SGD YCL050C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0008796bis(5'-nucleosyl)-tetraphosphatase activityTAS:SGD.
Biological processGO:0009117nucleotide metabolic processTAS:SGD.
EC number 2.7.7.53,2.7.7.5

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine (Probable).
60Phosphothreonine.
185Phosphothreonine.

Sequence Information
Sequence length321
Molecular weight36493

	---------+----------+----------+----------+----------+
MSIPADIASL ISDKYKSAFD NGNLKFIQTE TTKTKDPKTS MPYLISHMPS   50
LIEKPERGQT PEGEDPLGKP EEELTVIPEF GGADNKAYKL LLNKFPVIPE   100
HTLLVTNEYQ HQTDALTPTD LLTAYKLLCA LDNEESDKRH MVFYNSGPAS   150
GSSLDHKHLQ ILQMPEKFVT FQDRLCNGKE HFLPTFNTEP LQDAKVSFAH   200
FVLPMPESEE TVDEDLLAMC YISILQRALT FFQDWLNENP ELKKSYNLML   250
TKEWICVVPR SKAFSDEMKI GFNSTGYCGM ILTKNDEVFS KITEKPELIN   300
DILLECGFPN TSGQKPNEYN Y                                  321