SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAMN1
Systematic nameYBR158W
AliasCST13, ICS4
DescriptionAntagonist of mitotic exit network protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38285
SGD YBR158W
Gene Ontology (GO)
Cellular componentGO:0005933cellular budIDA:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:SGD.
Biological processGO:0007093mitotic checkpointIMP:SGD.
Biological processGO:0001100negative regulation of exit from mitosisIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length549
Molecular weight62686

	---------+----------+----------+----------+----------+
MKLERVSSNG SFKRGRDIQS LESPCTRPLK KMSPSPSFTS LKMEKPFKDI   50
VRKYGGHLHQ SSYNPGSSKV ELVRPDLSLK TDQSFLQSSV QTTPNKKSCN   100
EYLSTPEATP LKNTATENAW ATSRVVSASS LSIVTPTEIK NILVDEFSEL   150
KLGQPLTAQH QRSHAVFEIP EIVENIIKMI VSLESANIPK ERPCLRRNPQ   200
SYEHSLLMYK DEERAKKAWS AAQQLRDPPL VGHKEKKQGA LFSCMMVNRL   250
WLNVTRPFLF KSLHFKSVHN FKEFLRTSQE TTQVMRPSHF ILHKLHQVTQ   300
PDIERLSRME CQNLKWLEFY VCPRITPPLS WFDNLHKLEK LIIPGNKNID   350
DNFLLRLSQS IPNLKHLVLR ACDNVSDSGV VCIALNCPKL KTFNIGRHRR   400
GNLITSVSLV ALGKYTQVET VGFAGCDVDD AGIWEFARLN GKNVERLSLN   450
SCRLLTDYSL PILFALNSFP NLAVLEIRNL DKITDVRHFV KYNLWKKSLD   500
APILIEACER ITKLIDQEEN RVKRINSLVA LKDMTAWVNA DDEIENNVD    549