SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameALK1
Systematic nameYGL021W
DescriptionSerine/threonine-protein kinase Haspin homolog ALK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP43633
SGD YGL021W
Gene Ontology (GO)
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0007067mitosisIEP:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
172Phosphothreonine.
318Phosphoserine.

Sequence Information
Sequence length760
Molecular weight86087

	---------+----------+----------+----------+----------+
MLLHFDIVIQ LLSSHTLKSH QVEPPMDFET SFEEFVEDKR FIALEVSDND   50
DDCDTDLTAD TADELESSAI LKMRESDASL NVTTGNNTSR KTTSNSKKRW   100
SLLSNHSAVS SSKSKKRWSV LSSSFTSESH KDRESRNVLQ QKRKSLQSYS   150
SLDTVASNSS ISASSSLKRS STGLSLRQLF TKIGINDDIS QPGIGIPQGK   200
ENLPPTMGKK NSSIASTSSE NRLRTPLKPL VNHSKRPTSQ PQQQQPLYNA   250
SLSSRRSSIS STVSSSSSSK WRFWKRNKNQ TPALLQPDHH SLKTFPAVNR   300
RDSMTPVEPR NMVKHKTSFS DFHKTIFSSN TYSESSDTIS SMEITLKNKA   350
SSSSLSLNVL KKRNSQSSLK HKSSHASLQK FKRNKGKSSM IAPSTATNSS   400
NDDSCSYSSK NSTLSHRISL PVPDQVSRDK IQNKLRYSTS LLSLNSKSSL   450
PMNKNDHDET LLRQILLNCD IKRILNPAKG DVLPLINDVN HLSSIQLTSN   500
VWQIGEVICK KVSLGTIDDI TWDRKFLSLQ ELEKLKIMQQ KFDGIPQLLK   550
SFVVKEANGG LYLYLLFKDH GTPISLISLK NWKQILKIFW SCAGIIHGLE   600
KNLKFEHRNL TLDNILIDGN GNITIIDFKC SRLQTPQDDV LYLRLDHPLF   650
FLNGKDKSKI NEYQYQFEFE IYQSMRILLN MDASAFEPMT NLYWLYYLSR   700
VLLKFGDRKL GKNDANRDKM ARVINHLEMN LAVHKRGGQL FKRLETEDIK   750
NTGDLLKLYK                                                760