SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameALG2
Systematic nameYGL065C
DescriptionAlpha-1,3-mannosyltransferase ALG2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP43636
SGD YGL065C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumTAS:SGD.
Molecular functionGO:0004378glycolipid 3-alpha-mannosyltransferase acti...IDA:SGD.
Molecular functionGO:0033164glycolipid 6-alpha-mannosyltransferase acti...IDA:SGD.
Biological processGO:0006490oligosaccharide-lipid intermediate assemblyIDA:SGD.
EC number 2.4.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
302Phosphothreonine.

Sequence Information
Sequence length503
Molecular weight58047

	---------+----------+----------+----------+----------+
MIEKDKRTIA FIHPDLGIGG AERLVVDAAL GLQQQGHSVI IYTSHCDKSH   50
CFEEVKNGQL KVEVYGDFLP TNFLGRFFIV FATIRQLYLV IQLILQKKVN   100
AYQLIIIDQL STCIPLLHIF SSATLMFYCH FPDQLLAQRA GLLKKIYRLP   150
FDLIEQFSVS AADTVVVNSN FTKNTFHQTF KYLSNDPDVI YPCVDLSTIE   200
IEDIDKKFFK TVFNEGDRFY LSINRFEKKK DVALAIKAFA LSEDQINDNV   250
KLVICGGYDE RVAENVEYLK ELQSLADEYE LSHTTIYYQE IKRVSDLESF   300
KTNNSKIIFL TSISSSLKEL LLERTEMLLY TPAYEHFGIV PLEAMKLGKP   350
VLAVNNGGPL ETIKSYVAGE NESSATGWLK PAVPIQWATA IDESRKILQN   400
GSVNFERNGP LRVKKYFSRE AMTQSFEENV EKVIWKEKKY YPWEIFGISF   450
SNFILHMAFI KILPNNPWPF LFMATFMVLY FKNYLWGIYW AFVFALSYPY   500
EEI                                                      503