SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameALD6
Systematic nameYPL061W
AliasALDH1
DescriptionMagnesium-activated aldehyde dehydrogenase, cytosolic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K3        
2         
3 MULTI    RPN10 Proteasomal Degradation
4        Proteasomal Degradation
5         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[5]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP54115
SGD YPL061W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:00040283-chloroallyl aldehyde dehydrogenase activityIMP:SGD.
Biological processGO:0019413acetate biosynthetic processIMP:SGD.
EC number 1.2.1.3

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
491Phosphothreonine.

Sequence Information
Sequence length500
Molecular weight54414

	---------+----------+----------+----------+----------+
MTKLHFDTAE PVKITLPNGL TYEQPTGLFI NNKFMKAQDG KTYPVEDPST   50
ENTVCEVSSA TTEDVEYAIE CADRAFHDTE WATQDPRERG RLLSKLADEL   100
ESQIDLVSSI EALDNGKTLA LARGDVTIAI NCLRDAAAYA DKVNGRTINT   150
GDGYMNFTTL EPIGVCGQII PWNFPIMMLA WKIAPALAMG NVCILKPAAV   200
TPLNALYFAS LCKKVGIPAG VVNIVPGPGR TVGAALTNDP RIRKLAFTGS   250
TEVGKSVAVD SSESNLKKIT LELGGKSAHL VFDDANIKKT LPNLVNGIFK   300
NAGQICSSGS RIYVQEGIYD ELLAAFKAYL ETEIKVGNPF DKANFQGAIT   350
NRQQFDTIMN YIDIGKKEGA KILTGGEKVG DKGYFIRPTV FYDVNEDMRI   400
VKEEIFGPVV TVAKFKTLEE GVEMANSSEF GLGSGIETES LSTGLKVAKM   450
LKAGTVWINT YNDFDSRVPF GGVKQSGYGR EMGEEVYHAY TEVKAVRIKL   500

	* Ubiquitinated lysine is denoted as red color.