SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameALA1
Systematic nameYOR335C
DescriptionAlanyl-tRNA synthetase, cytoplasmic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40825
SGD YOR335C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004813alanine-tRNA ligase activityIGI:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006419alanyl-tRNA aminoacylationIGI:SGD.
EC number 6.1.1.7

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
950Phosphoserine.

Sequence Information
Sequence length958
Molecular weight107277

	---------+----------+----------+----------+----------+
MTIGDKQKWT ATNVRNTFLD YFKSKEHKFV KSSPVVPFDD PTLLFANAGM   50
NQYKPIFLGT VDPASDFYTL KRAYNSQKCI RAGGKHNDLE DVGKDSYHHT   100
FFEMLGNWSF GDYFKKEAIT YSWTLLTEVY GIPKDRLYVT YFEGDEKLGL   150
EPDTEARELW KNVGVPDDHI LPGNAKDNFW EMGDQGPCGP CSEIHYDRIG   200
GRNAASLVNM DDPDVLEVWN LVFIQFNREQ DGSLKPLPAK HIDTGMGFER   250
LVSVLQDVRS NYDTDVFTPL FERIQEITSV RPYSGNFGEN DKDGIDTAYR   300
VLADHVRTLT FALADGGVPN NEGRGYVLRR ILRRGARYAR KYMNYPIGNF   350
FSTLAPTLIS QVQDIFPELA KDPAFLFEIL DEEEASFAKT LDRGERLFEK   400
YASAASKTES KTLDGKQVWR LYDTYGFPVD LTELMAEEQG LKIDGPGFEK   450
AKQESYEASK RGGKKDQSDL IKLNVHELSE LNDAKVPKTN DEFKYGSANV   500
EGTILKLHDG TNFVDEITEP GKKYGIILDK TCFYAEQGGQ EYDTGKIVID   550
DAAEFNVENV QLYNGFVFHT GSLEEGKLSV GDKIIASFDE LRRFPIKNNH   600
TGTHILNFAL KETLGNDVDQ KGSLVAPEKL RFDFSHKKAV SNEELKKVED   650
ICNEQIKENL QVFYKEIPLD LAKSIDGVRA VFGETYPDPV RVVSVGKPIE   700
ELLANPANEE WTKYSIEFCG GTHVNKTGDI KYFVILEESG IAKGIRRIVA   750
VTGTEAFEAQ RLAEQFAADL DAADKLPFSP IKEKKLKELG VKLGQLSISV   800
ITKNELKQKF NKIEKAVKDE VKSRAKKENK QTLDEVKTFF ETNENAPYLV   850
KFIDISPNAK AITEAINYMK SNDSVKDKSI YLLAGNDPEG RVAHGCYISN   900
AALAKGIDGS ALAKKVSSII GGKAGGKGNV FQGMGDKPAA IKDAVDDLES   950
LFKEKLSI                                                 958