SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAKL1
Systematic nameYBR059C
DescriptionSerine/threonine-protein kinase AKL1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K1008;K1046        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38080
SGD YBR059C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0030036actin cytoskeleton organization and biogenesisIMP:SGD.
Biological processGO:0030100regulation of endocytosisIMP:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
504Phosphoserine.
518Phosphoserine.
521Phosphoserine.
541Phosphoserine.
801Phosphoserine.
879Phosphoserine.
884Phosphoserine.
960Phosphoserine.
1048Phosphoserine.
1051Phosphoserine.
1072Phosphoserine.

Sequence Information
Sequence length1108
Molecular weight123989

	---------+----------+----------+----------+----------+
MSITNGTSRS VSAMGHPAVE RYTPGHIVCV GTHKVEVVNY LAEGGFAQIY   50
VVKFLEYLNE FDNTASVPLK IGDVACLKRV LVQDENGLNE MRNEVEVMKK   100
LKGAPNIVQY FDSNASRRRD GVQGFEVLLL MELCPNKSLL DYMNQRLSTK   150
LTEAEIVKIM YDVALSISQM HYLPVSLIHR DIKIENVLVD AKNNFKLADF   200
GSTSTCFPIV TTHQDIALLT QNIYVHTTPQ YRSPEMIDLY RCLPINEKSD   250
IWALGVFLYK LLFFTTPFEM TGQFAILHSK YEFPVNKYSS KLINLIIIML   300
AENPNLRPNI YQVLYHLCEI LNVEVPIEDK YAEGAYNFSK YTQFQNKLQN   350
VQLQMYQLQQ KKIMQNNKLS DSEENLLNDM FLSSFEISSK LPMNASDGHA   400
AVSRIPSQNV GQELEEEKES QSDQRKSTLS EDKSSRTTSN ANSSGTANNP   450
QEINTIQSPG IEDKSIFENK TPGELYYPSV SELDTYLDKE LVKQSSDPTI   500
SEQSPRLNTQ SLPQRQKSTS SYSSGGRSMK STSYGAATIG SDEALANEKT   550
AGINKMKQHK SNNPFPKMNV AYHSTNELSN DASNFFLEEQ QQGQRYQQAQ   600
NQTGTQGNTF PDESQYQSRV EQQQQQQDQP KGPANYSQRN FYTGRDRSNK   650
PMQLGGTIAG DSGNRRVNFQ NISQNYATNS QSGYLPSQNS PAIPMVRPVI   700
SMNQQQAQQI QAQQLQAQQM QAKQQMQAKQ QMQVQQQLQV QQQMQIQNAN   750
NNGTYVSDRT NHTTEDMRNA QGGEPPILAG NSANEPMHSS SKNEALLIEL   800
SPLKEDAGKQ SFQDTNEPQT GGIEDAGGSG TIKGSNNNRN GVLNLSLNEM   850
DLSRDDTGAA VSSFSSSSSS ASIQQAKLSG RKGSSKRNNY STDELGDSMV   900
SSESIDIDLD DARRGKTAER RPLHNERGHK DQARSSDASK SNQFKSKDFS   950
SVSTRQPRQS LDLNFQEVNL SSPTLTQEHR NKNDSPAPNS HHSYRVSPHA   1000
STAITENKRH STGHELSTRS NGKHETHRTG SKQRHDLERY RHSKDKDSNS   1050
SITISTSTPS EMRKSFARAR QSLDLERVRR EAMASSASSS GGSNGKRRSF   1100
FSVFRSEK                                                 1108

	* Ubiquitinated lysine is denoted as red color.