SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAGP1
Systematic nameYCL025C
DescriptionGeneral amino acid permease AGP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25376
SGD YCL025C
Gene Ontology (GO)
Molecular functionGO:0005296L-proline permease activityIGI:SGD.
Biological processGO:0006865amino acid transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length595
Molecular weight64866

	---------+----------+----------+----------+----------+
MSSSKSLYEL KDLKNSSTEI HATGQDNEIE YFETGSNDRP SSQPHLGYEQ   50
HNTSAVRRFF DSFKRADQGP QDEVEATQMN DLTSAISPSS RQAQELEKNE   100
SSDNIGANTG HKSDSLKKTI QPRHVLMIAL GTGIGTGLLV GNGTALVHAG   150
PAGLLIGYAI MGSILYCIIQ ACGEMALVYS NLTGGYNAYP SFLVDDGFGF   200
AVAWVYCLQW LCVCPLELVT ASMTIKYWTT SVNPDVFVII FYVLVITINI   250
FGARGYAEAE FFFNCCKILM MTGFFILGII IDVGGAGNDG FIGGKYWHDP   300
GAFNGKHAID RFKGVAATLV TAAFAFGGSE FIAITTAEQS NPRKAIPGAA   350
KQMIYRILFL FLATIILLGF LVPYNSDQLL GSTGGGTKAS PYVIAVASHG   400
VRVVPHFINA VILLSVLSMA NSSFYSSARL FLTLSEQGYA PKVFSYIDRA   450
GRPLIAMGVS ALFAVIAFCA ASPKEEQVFT WLLAISGLSQ LFTWTAICLS   500
HLRFRRAMKV QGRSLGELGF KSQTGVWGSA YACIMMILIL IAQFWVAIAP   550
IGEGKLDAQA FFENYLAMPI LIALYVGYKV WHKDWKLFIR ETRST        595