SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAFT2
Systematic nameYPL202C
DescriptionIron-regulated transcriptional activator AFT2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08957
SGD YPL202C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIMP:SGD.
Molecular functionGO:0003704specific RNA polymerase II transcription fa...IMP:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Biological processGO:0006879iron ion homeostasisIMP:SGD.
Biological processGO:0045941positive regulation of transcriptionIDA:SGD.
Biological processGO:0006979response to oxidative stressIMP:SGD.
Biological processGO:0006367transcription initiation from RNA polymeras...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length416
Molecular weight47105

	---------+----------+----------+----------+----------+
MKAKSMKSII SVPISVSKTG KMKLTASPDN LASMMSKDQN KLIHLDPVPS   50
FEDRHEIKPW LQKIFYPQGI DIVIERSDSS KVTFKCRSVR SKVGLNPKSK   100
GSSSRSHACP FRIRAAYSVR LQKWNVVVMN NIHSHELRFD LITKTDDYKK   150
FKENLRQKND EKAIKTFDEL EYKASLNLPL VTPIISCDCG LTKEIEAFNN   200
IFLPLSNPPL TSKKNLLKTN KNSVSKIKSR QMDNSKPRPR LKTKLDADLH   250
DTGFLDNFKT RNSCVKIEKE DSLTNLNEID FTNMFCNDNF IQNYNQGLME   300
LLTEPTPGPS SSSCILPSTP TRPLSQSKMD IALSESTTSS PNFMETDAPY   350
GDEIIKVSKD TKSNAPTADT DIATNLGKER NENFGMLNYN YEALLHFNDE   400
HFNELNSIDP ALISKY                                        416