SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameADE3
Systematic nameYGR204W
DescriptionFormyltetrahydrofolate synthetase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP07245
SGD YGR204W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004329formate-tetrahydrofolate ligase activityIMP:SGD.
Molecular functionGO:0004477methenyltetrahydrofolate cyclohydrolase act...IMP:SGD.
Molecular functionGO:0004488methylenetetrahydrofolate dehydrogenase (NA...IMP:SGD.
Biological processGO:0009113purine base biosynthetic processIMP:SGD.
EC number 1.5.1.5,3.5.4.9,6.3.4.3

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length946
Molecular weight102205

	---------+----------+----------+----------+----------+
MAGQVLDGKA CAQQFRSNIA NEIKSIQGHV PGFAPNLAII QVGNRPDSAT   50
YVRMKRKAAE EAGIVANFIH LDESATEFEV LRYVDQLNED PHTHGIIVQL   100
PLPAHLDEDR ITSRVLAEKD VDGFGPTNIG ELNKKNGHPF FLPCTPKGII   150
ELLHKANVTI EGSRSVVIGR SDIVGSPVAE LLKSLNSTVT ITHSKTRDIA   200
SYLHDADIVV VAIGQPEFVK GEWFKPRDGT SSDKKTVVID VGTNYVADPS   250
KKSGFKCVGD VEFNEAIKYV HLITPVPGGV GPMTVAMLMQ NTLIAAKRQM   300
EESSKPLQIP PLPLKLLTPV PSDIDISRAQ QPKLINQLAQ ELGIYSHELE   350
LYGHYKAKIS PKVIERLQTR QNGKYILVSG ITPTPLGEGK STTTMGLVQA   400
LTAHLGKPAI ANVRQPSLGP TLGVKGGAAG GGYSQVIPMD EFNLHLTGDI   450
HAIGAANNLL AAAIDTRMFH ETTQKNDATF YNRLVPRKNG KRKFTPSMQR   500
RLNRLGIQKT NPDDLTPEEI NKFARLNIDP DTITIKRVVD INDRMLRQIT   550
IGQAPTEKNH TRVTGFDITV ASELMAILAL SKDLRDMKER IGRVVVAADV   600
NRSPVTVEDV GCTGALTALL RDAIKPNLMQ TLEGTPVLVH AGPFANISIG   650
ASSVIADRVA LKLVGTEPEA KTEAGYVVTE AGFDFTMGGE RFFNIKCRSS   700
GLTPNAVVLV ATVRALKSHG GAPDVKPGQP LPSAYTEENI EFVEKGAANM   750
CKQIANIKQF GVPVVVAINK FETDTEGEIA AIRKAALEAG AFEAVTSNHW   800
AEGGKGAIDL AKAVIEASNQ PVDFHFLYDV NSSVEDKLTT IVQKMYGGAA   850
IDILPEAQRK IDMYKEQGFG NLPICIAKTQ YSLSHDATLK GVPTGFTFPI   900
RDVRLSNGAG YLYALAAEIQ TIPGLATYAG YMAVEVDDDG EIDGLF       946