SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameACT1
Systematic nameYFL039C
AliasABY1, END7
DescriptionActin

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP60010
SGD YFL039C
Gene Ontology (GO)
Cellular componentGO:0030482actin cableTAS:SGD.
Cellular componentGO:0030479actin cortical patchTAS:SGD.
Cellular componentGO:0000142cellular bud neck contractile ringTAS:SGD.
Cellular componentGO:0031011INO80 complexIPI:SGD.
Cellular componentGO:0035267NuA4 histone acetyltransferase complexIPI:SGD.
Cellular componentGO:0000812SWR1 complexIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:UniProtKB.
Biological processGO:0007119budding cell isotropic bud growthTAS:SGD.
Biological processGO:0007047cell wall organization and biogenesisTAS:SGD.
Biological processGO:0001300chronological cell agingIMP:SGD.
Biological processGO:0000916cytokinesis, contractile ring contractionIDA:SGD.
Biological processGO:0006281DNA repairIDA:SGD.
Biological processGO:0006897endocytosisTAS:SGD.
Biological processGO:0000132establishment of mitotic spindle orientationTAS:SGD.
Biological processGO:0006887exocytosisTAS:SGD.
Biological processGO:0016573histone acetylationIDA:SGD.
Biological processGO:0000001mitochondrion inheritanceTAS:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IDA:SGD.
Biological processGO:0006970response to osmotic stressTAS:SGD.
Biological processGO:0006979response to oxidative stressIGI:SGD.
Biological processGO:0000011vacuole inheritanceIGI:SGD.
Biological processGO:0030050vesicle transport along actin filamentTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1N-acetylmethionine.

Sequence Information
Sequence length375
Molecular weight41690

	---------+----------+----------+----------+----------+
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK   50
DSYVGDEAQS KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE   100
HPVLLTEAPM NPKSNREKMT QIMFETFNVP AFYVSIQAVL SLYSSGRTTG   150
IVLDSGDGVT HVVPIYAGFS LPHAILRIDL AGRDLTDYLM KILSERGYSF   200
STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY ELPDGQVITI   250
GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS   300
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT   350
TFQQMWISKQ EYDESGPSIV HHKCF                              375