SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameACS2
Systematic nameYLR153C
DescriptionAcetyl-coenzyme A synthetase 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K506        
2         
3 MULTI       
4        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP52910
SGD YLR153C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0003987acetate-CoA ligase activityIDA:SGD.
Biological processGO:0006085acetyl-CoA biosynthetic processIDA:SGD.
Biological processGO:0016573histone acetylationIMP:SGD.
EC number 6.2.1.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length683
Molecular weight75492

	---------+----------+----------+----------+----------+
MTIKEHKVVY EAHNVKALKA PQHFYNSQPG KGYVTDMQHY QEMYQQSINE   50
PEKFFDKMAK EYLHWDAPYT KVQSGSLNNG DVAWFLNGKL NASYNCVDRH   100
AFANPDKPAL IYEADDESDN KIITFGELLR KVSQIAGVLK SWGVKKGDTV   150
AIYLPMIPEA VIAMLAVARI GAIHSVVFAG FSAGSLKDRV VDANSKVVIT   200
CDEGKRGGKT INTKKIVDEG LNGVDLVSRI LVFQRTGTEG IPMKAGRDYW   250
WHEEAAKQRT YLPPVSCDAE DPLFLLYTSG STGSPKGVVH TTGGYLLGAA   300
LTTRYVFDIH PEDVLFTAGD VGWITGHTYA LYGPLTLGTA SIIFESTPAY   350
PDYGRYWRII QRHKATHFYV APTALRLIKR VGEAEIAKYD TSSLRVLGSV   400
GEPISPDLWE WYHEKVGNKN CVICDTMWQT ESGSHLIAPL AGAVPTKPGS   450
ATVPFFGINA CIIDPVTGVE LEGNDVEGVL AVKSPWPSMA RSVWNHHDRY   500
MDTYLKPYPG HYFTGDGAGR DHDGYYWIRG RVDDVVNVSG HRLSTSEIEA   550
SISNHENVSE AAVVGIPDEL TGQTVVAYVS LKDGYLQNNA TEGDAEHITP   600
DNLRRELILQ VRGEIGPFAS PKTIILVRDL PRTRSGKIMR RVLRKVASNE   650
AEQLGDLTTL ANPEVVPAII SAVENQFFSQ KKK                     683

	* Ubiquitinated lysine is denoted as red color.