SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameACK1
Systematic nameYDL203C
DescriptionActivator of C kinase protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K184        
2 MULTI HECTNARSP5   
3   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidenceUb-conjugate Confirmation
Methodluminescent assay (biotin-ubiquitin, GST-substrate)
Reference"A high throughput screen to identify substrates for the ubiquitin ligase Rsp5."
Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, Lee T, Chan D, Wolting C, Edwards A, Bosse R, Rotin D.
J Biol Chem. 280(33):29470-8 (2005) [15955809]
[3]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionQ07622
SGD YDL203C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
143Phosphoserine.

Sequence Information
Sequence length623
Molecular weight69371

	---------+----------+----------+----------+----------+
MVNQGQPQPN LYDKHINMFP PARARESSHK LGNANSDRHG LPAQNIVPAP   50
YPVDDSIVEL TPAIPFTSPS SSSSLSLPLS ALNFTDGNAD GGQLGTPVTI   100
NSNNGMDIFN SKPTGEIGYA NNGTNSTGSR YELPFNFSST KESLGSPAVQ   150
DASISSGNRI SESVRDNSAP PPYEESESRI LQEKVYRTEE KAPIRPLNNN   200
PVPPQKINQP PTGSAKTDDN GSSGGEDKLS SYSPEALAFY QVYKKTITDS   250
SKFTPEIQMQ WCETLLTYAF NEDFISQYNI NAEKLKRSLK PEEMLKNQKV   300
ILEHSFKVLT KLITLKWPPA MYLMGTLYSH QPYLPIKNKN IVIKNDEKAL   350
EYYCKAAKLN NSDACYRAGV CFEYQRGTSS LDPSPTKEQC IKKAFQYYQH   400
GAEVCSNSAC MYKLGMSHLY GLNMQKTDVL LAIKWFDKAA QKGDSPQTLY   450
ELGKIYEFSV LPPEIQNLLF ANGIRKDSQL AIKYYQQCAK DFEYPLAQWK   500
LGNCYEFGDL GLPVVAKKSI YWYSKAAAAQ PKGNPMAMLS LSGWYLTGAP   550
NILKPNNKEA FNWALKSSKC SDGKLARTEF ALGFYYEKGV GCEVDLDLAK   600
QYYQRAARMG FRKAVDALRS LTN                                623

	* Ubiquitinated lysine is denoted as red color.