SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameABP1
Systematic nameYCR088W
DescriptionActin-binding protein

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP15891
SGD YCR088W
Gene Ontology (GO)
Cellular componentGO:0005938cell cortexIDA:SGD.
Cellular componentGO:0005856cytoskeletonTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000147actin cortical patch assemblyIMP:SGD.
Biological processGO:0030468establishment of cell polarity (sensu Fungi)IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine.
157Phosphothreonine.
165Phosphothreonine.
167Phosphoserine.
169Phosphoserine.
181Phosphothreonine.
183Phosphoserine.
365Phosphoserine.
389Phosphoserine.
458Phosphoserine.
478Phosphoserine.
481Phosphoserine.

Sequence Information
Sequence length592
Molecular weight65576

	---------+----------+----------+----------+----------+
MALEPIDYTT HSREIDAEYL KIVRGSDPDT TWLIISPNAK KEYEPESTGS   50
SFHDFLQLFD ETKVQYGLAR VSPPGSDVEK IIIIGWCPDS APLKTRASFA   100
ANFAAVANNL FKGYHVQVTA RDEDDLDENE LLMKISNAAG ARYSIQTSSK   150
QQGKASTPPV KKSFTPSKSP APVSKKEPVK TPSPAPAAKI SSRVNDNNDD   200
DDWNEPELKE RDFDQAPLKP NQSSYKPIGK IDLQKVIAEE KAKEDPRLVQ   250
KPTAAGSKID PSSDIANLKN ESKLKRDSEF NSFLGTTKPP SMTESSLKND   300
DDKVIKGFRN EKSPAQLWAE RKAKQNSGNA ETKAEAPKPE VPEDEPEGEP   350
DVKDLKSKFE GLAASEKEEE EMENKFAPPP KKSEPTIISP KPFSKPQEPV   400
KAEEAEQPKT DYKKIGNPLP GMHIEADNEE EPEENDDDWD DDEDEAAQPP   450
LPSRNVASGA PVQKEEPEQE EIAPSLPSRN SIPAPKQEEA PEQAPEEEIE   500
EEAEEAAPQL PSRSSAAPPP PPRRATPEKK PKENPWATAE YDYDAAEDNE   550
LTFVENDKII NIEFVDDDWW LGELEKDGSK GLFPSNYVSL GN           592