SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameABD1
Systematic nameYBR236C
DescriptionmRNA cap guanine-N7 methyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32783
SGD YBR236C
Gene Ontology (GO)
Cellular componentGO:0016591DNA-directed RNA polymerase II, holoenzymeIPI:SGD.
Molecular functionGO:0004482mRNA (guanine-N7-)-methyltransferase activityIDA:SGD.
Biological processGO:0006370mRNA cappingIDA:SGD.
EC number 2.1.1.56

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length436
Molecular weight50341

	---------+----------+----------+----------+----------+
MSTKPEKPIW MSQEDYDRQY GSITGDESST VSKKDSKVTA NAPGDGNGSL   50
PVLQSSSILT SKVSDLPIEA ESGFKIQKRR HERYDQEERL RKQRAQKLRE   100
EQLKRHEIEM TANRSINVDQ IVREHYNERT IIANRAKRNL SPIIKLRNFN   150
NAIKYMLIDK YTKPGDVVLE LGCGKGGDLR KYGAAGISQF IGIDISNASI   200
QEAHKRYRSM RNLDYQVVLI TGDCFGESLG VAVEPFPDCR FPCDIVSTQF   250
CLHYAFETEE KARRALLNVA KSLKIGGHFF GTIPDSEFIR YKLNKFPKEV   300
EKPSWGNSIY KVTFENNSYQ KNDYEFTSPY GQMYTYWLED AIDNVPEYVV   350
PFETLRSLAD EYGLELVSQM PFNKFFVQEI PKWIERFSPK MREGLQRSDG   400
RYGVEGDEKE AASYFYTMFA FRKVKQYIEP ESVKPN                  436