SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameAAH1
Systematic nameYNL141W
DescriptionAdenosine deaminase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2  CDC34RINGSCFSAF1  Proteasomal Degradation
3K329  RINGSCFSAF1   
4         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Proteasome- and SCF-dependent degradation of yeast adenine deaminase upon transition from proliferation to quiescence requires a new F-box protein named Saf1p."
Escusa S, Camblong J, Galan JM, Pinson B, Daignan-Fornier B.
Mol Microbiol. 60(4):1014-25 (2006) [16677311]
[3]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Skp1-Cullin-F-box-dependent degradation of Aah1p requires its interaction with the F-box protein Saf1p."
Escusa S, Laporte D, Massoni A, Boucherie H, Dautant A, Daignan-Fornier B.
J Biol Chem. 282(28):20097-103 (2007) [17517885]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53909
SGD YNL141W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0000034adenine deaminase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number 3.5.4.4

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length347
Molecular weight39635

	---------+----------+----------+----------+----------+
MVSVEFLQEL PKCEHHLHLE GTLEPDLLFP LAKRNDIILP EGFPKSVEEL   50
NEKYKKFRDL QDFLDYYYIG TNVLISEQDF FDLAWAYFKK VHKQGLVHAE   100
VFYDPQSHTS RGISIETVTK GFQRACDKAF SEFGITSKLI MCLLRHIEPE   150
ECLKTIEEAT PFIKDGTISA LGLDSAEKPF PPHLFVECYG KAASLNKDLK   200
LTAHAGEEGP AQFVSDALDL LQVTRIDHGI NSQYDEELLD RLSRDQTMLT   250
ICPLSNVKLQ VVQSVSELPL QKFLDRDVPF SLNSDDPAYF GGYILDVYTQ   300
VSKDFPHWDH ETWGRIAKNA IKGSWCDDKR KNGLLSRVDE VVTKYSH      347

	* Ubiquitinated lysine is denoted as red color.